Exosome separation by Aqueous two phase system (ATPS)

EVs are isolated by a variety of methods, such as ultracentrifugation (U/C), size exclusion chromatography and immunoaffinity. These methods commonly involve a lengthy and complicated process with low yield, so they have compromised the utility of EVs for clinical diagnosis. The use of aqueous two-phase systems (ATPS) can overcome these difficulties of isolating EVs by using chemical properties between EVs and polymers. This method provides high EV recovery efficiency in a short time. Consequently, it can significantly increase the diagnostic applicability of EV.

Reference

Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis, PLoS One, 2018